Abstract
Prions have been extensively studied since they represent a new class of infectious agents, the pathogenic prion protein (PrPSc). However, a central question on the physiological function of the normal prion protein (PrPC) remains unresolved. A cell model which was previously established from Rikn mice (PrP-/-) remains problematic because of its ectopic expression of the doppel (Dpl) which may have a neurotoxic effect. Here we established neuronal cell lines from Zürich I (PrP-/-) which do not express Dpl protein and ICR mice (PrP+/+) by transfecting with plasmid encoding for the large T antigen of SV40. The transformed cells have shown neuronal characteristics and, thus, these cell lines may provide a useful model to explore the function of neuronal PrPC.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western / methods
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Cell Count / methods
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Cell Cycle / physiology
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Cell Line, Transformed / metabolism
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Cell Size
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Cells, Cultured
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Embryo, Mammalian
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Fluorescent Antibody Technique / methods
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GPI-Linked Proteins
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Hippocampus / cytology*
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Mice
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Mice, Inbred C57BL
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Mice, Inbred ICR
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Mice, Knockout
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Microtubule-Associated Proteins / metabolism
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Neurons / metabolism*
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Phosphopyruvate Hydratase / metabolism
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PrPC Proteins / genetics
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PrPC Proteins / metabolism*
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Prions / metabolism*
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RNA, Messenger / biosynthesis
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Time Factors
Substances
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GPI-Linked Proteins
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Microtubule-Associated Proteins
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Mtap2 protein, mouse
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PrPC Proteins
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Prions
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Prnd protein, mouse
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RNA, Messenger
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Phosphopyruvate Hydratase