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Guang Pu Xue Yu Guang Pu Fen Xi. 2004 Nov;24(11):1373-5.

[TLC-FT-SERS study on a pair of optic isomers in ephedra].

[Article in Chinese]

Author information

  • 1Department of Chemistry, Capital Normal University, Beijing 100037, China.

Abstract

A new method for analyzing the ingredients of a pair of optic isomers in ephedra, nor-ephedrine and nor-pseudo-ephedrine, using hyphenated high-efficiency thin layer chromatography (TLC) and surface-enhanced Raman spectroscopy (SERS) techniques, is reported. The results show that the characteristic spectral bands of nor-ephedrine and nor-pseudo-ephedrine can be obtained from the TLC spot with 8 microg sample of about 2.0 mm in diameter. The difference between the SERS and solid spectra was found. Spectral bands at 1004 cm(-1) and 1605 cm(-1) were found greatly enhanced. Molecule was absorbed in surface silver sol by pi electrons in ring. Under similar experimental conditions the spectral information of Levo-nor-ephedrine ramifications TLC-SERS is rich with strong credibility, whereas dextral-nor-ephedrine ramifications show a relatively strong fluorescence backdrop with less spectral information and weak credibility. The effective combination of TLC and SERS can be used to analyse the chemical ingredients with high sensitivity.

PMID:
15762480
[PubMed - indexed for MEDLINE]
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