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    J Bacteriol. 2005 Mar;187(6):2215-7.

    Kinetic characterization of the glycosyltransferase module of Staphylococcus aureus PBP2.

    Barrett D, Leimkuhler C, Chen L, Walker D, Kahne D, Walker S.

    Source

    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.

    Abstract

    We report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes.

    PMID:
    15743972
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC1064046
    Free PMC Article

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