Format

Send to:

Choose Destination
See comment in PubMed Commons below
Mycol Res. 2005 Jan;109(Pt 1):115-24.

Screening of basidiomycetes and xylariaceous fungi for lignin peroxidase and laccase gene-specific sequences.

Author information

  • 1Department of Ecology and Biodiversity, University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China. pointing@hkucc.hku.hk

Abstract

Lignin peroxidase and laccase gene-specific PCR primers were used to screen 38 diverse basidiomycetes and xylariaceous fungi. Lignin peroxidase gene-specific sequences were obtained for basidiomycetes only and were highly divergent. Possession of laccase genes was relatively widespread among basidiomycetes, and is shown for the first time in Xylariaceae. All sequences were highly conserved with no variation resulting in changes to predicted amino acid sequence. Those basidiomycetes shown to possess lignin peroxidase and laccase genes also produced the enzyme in vitro. Conversely none of the xylariaceous fungi shown to possess laccase genes were able to do so, whilst others decolorized Poly R yet yielded no PCR amplicons.

PMID:
15736869
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk