Methods. 2005 Mar;35(3):248-55. Epub 2005 Jan 12.

Protein identification using 2D-LC-MS/MS.

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Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

Abstract

Multidimensional liquid chromatography techniques have been coupled to tandem mass spectrometry to provide a robust method to identify proteins in complex mixtures. Data acquisition is interfaced directly with search algorithms for identification through cross-correlation with databases. This review describes the most recent advances in methodologies for protein identification by mass spectrometry and describes the limitations of the application of the technologies.

PMID:: 15722221; [PubMed - indexed for MEDLINE]

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