Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Curr Opin Biotechnol. 2005 Feb;16(1):3-12.

From micro to nano: recent advances in high-resolution microscopy.

Author information

  • 1Delft University of Technology, Lorentzweg 1, 2628 CJ, Delft, The Netherlands. y.garini@tnw.tudelft.nl

Abstract

Improving the spatial resolution of optical microscopes is important for a vast number of applications in the life sciences. Optical microscopy allows intact samples and living cells to be studied in their natural environment, tasks that are not possible with other microscopy methods (e.g. electron microscopy). Major advances in the past two decades have significantly improved microscope resolution. By using interference and structured light methods microscope resolution has been improved to approximately 100 nm, and with non-linear methods a ten times improvement has been demonstrated to a current resolution limit of approximately 30 nm. These methods bring together old theoretical concepts such as interference with novel non-linear methods that improve spatial resolution beyond the limits that were previously assumed to be unreachable.

PMID:
15722009
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk