Biochem Biophys Res Commun. 2005 Mar 25;328(4):987-94.

Positional effects of the central DNA flap in HIV-1-derived lentiviral vectors.

Source

Laboratory for Molecular Virology and Gene Therapy, KULeuven and KULAK, Flanders, Belgium.

Abstract

During reverse transcription of the human immunodeficiency virus type 1 (HIV-1), a 90- or 99-nucleotide long DNA flap is formed at the centre of the viral cDNA. The presence of a central DNA flap in lentiviral vectors improves transduction efficiency significantly. We analysed the stimulation of lentiviral vector transduction by a DNA flap present at ectopic positions in the viral cDNA. A HIV-1 vector containing the cPPT/CTS fragment immediately downstream of the 5'-LTR performed as well as the wild-type cPPT-vector. Cloning of the cPPT/CTS fragment in front of the 3'-LTR resulted as well in a vector with higher transduction efficiency than a vector without central flap. These results demonstrate that the position of the DNA flap is not essential for its function in the context of HIV-1-derived lentiviral vectors. This may have consequences for vector design and our understanding of the functioning of the HIV-1 DNA flap.

PMID:: 15707975; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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DNA, Viral

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