HIV-1 Tat interacts with LIS1 protein

Retrovirology. 2005 Feb 7:2:6. doi: 10.1186/1742-4690-2-6.

Abstract

Background: HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization.

Results: We identified here LIS1 as a Tat-interacting protein during extensive biochemical fractionation of T-cell extracts. We found several proteins to co-purify with a Tat-associated RNAPII CTD kinase activity including LIS1, CDK7, cyclin H, and MAT1. Tat interacted with LIS1 but not with CDK7, cyclin H or MAT1 in vitro. LIS1 also co-immunoprecipitated with Tat expressed in HeLa cells. Further, LIS1 interacted with Tat in a yeast two-hybrid system.

Conclusion: Our results indicate that Tat interacts with LIS1 in vitro and in vivo and that this interaction might contribute to the effect of Tat on microtubule formation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-Alkyl-2-acetylglycerophosphocholine Esterase
  • Cyclin H
  • Cyclin-Dependent Kinase-Activating Kinase
  • Cyclin-Dependent Kinases / metabolism
  • Cyclins / metabolism
  • Gene Products, tat / metabolism*
  • HIV-1 / metabolism
  • HIV-1 / pathogenicity
  • HeLa Cells
  • Humans
  • Jurkat Cells
  • Microtubule-Associated Proteins / metabolism*
  • RNA Polymerase II / metabolism
  • Two-Hybrid System Techniques
  • tat Gene Products, Human Immunodeficiency Virus

Substances

  • CCNH protein, human
  • Cyclin H
  • Cyclins
  • Gene Products, tat
  • Microtubule-Associated Proteins
  • tat Gene Products, Human Immunodeficiency Virus
  • Cyclin-Dependent Kinases
  • RNA Polymerase II
  • 1-Alkyl-2-acetylglycerophosphocholine Esterase
  • PAFAH1B1 protein, human
  • Cyclin-Dependent Kinase-Activating Kinase