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1: Genome Biol. 2005;6(2):R19. Epub 2005 Jan 17.Click here to read Click here to read Links

A universal method for automated gene mapping.

Zipperlen P, Nairz K, Rimann I, Basler K, Hafen E, Hengartner M, Hajnal A.

Institute of Molecular Biology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland. peder.zipperlen@molbio.unizh.ch

Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual input and at comparatively low cost.

PMID: 15693948 [PubMed - indexed for MEDLINE]

PMCID: PMC551539