Rapid and simple quantification of bacterial cells by using a microfluidic device

Chieko Sakamoto; Nobuyasu Yamaguchi; Masao Nasu

doi:10.1128/AEM.71.2.1117-1121.2005

Rapid and simple quantification of bacterial cells by using a microfluidic device

Appl Environ Microbiol. 2005 Feb;71(2):1117-21. doi: 10.1128/AEM.71.2.1117-1121.2005.

Authors

Chieko Sakamoto¹, Nobuyasu Yamaguchi, Masao Nasu

Affiliation

¹ Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.

Abstract

This study investigated a microfluidic chip-based system (on-chip flow cytometry) for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count. The time required for this on-chip flow cytometry was only 30 min per 6 samples.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques
Colony Count, Microbial
Culture Media
Escherichia coli O157 / isolation & purification*
Flow Cytometry / instrumentation
Flow Cytometry / methods*
Microfluidics / instrumentation*
Microscopy, Fluorescence
Organic Chemicals
Pseudomonas putida / isolation & purification*
Rivers / microbiology
Staining and Labeling / methods
Time Factors

Substances

Culture Media
Organic Chemicals
SYBR Green II