J Med Chem. 2005 Feb 10;48(3):658-60.

Structure of human cytidine deaminase bound to a potent inhibitor.

Source

Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.

Abstract

Human cytidine deaminase (CDA) is an enzyme prominent for its role in catalyzing metabolic processing of nucleoside-type anticancer and antiviral agents. It is thus a promising target for the development of small molecule therapeutic adjuvants. We report the first crystal structure of human CDA as a complex with a tight-binding inhibitor, diazepinone riboside 1. The structure reveals that inhibitor 1 is able to establish a canonical pi/pi-interaction with a key active site residue, Phe 137.

PMID:: 15689149; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances, Secondary Source ID

MeSH Terms

Substances

Secondary Source ID

PDB/1MQ0

LinkOut - more resources

Full Text Sources

Chemical Information

BindingDB

Supplemental Content

Save items

Related citations in PubMed

Synthesis and conformational analysis of locked carbocyclic analogues of 1,3-diazepinone riboside, a high-affinity cytidine deaminase inhibitor. [J Org Chem. 2009]
Synthesis and conformational analysis of locked carbocyclic analogues of 1,3-diazepinone riboside, a high-affinity cytidine deaminase inhibitor.
Ludek OR, Schroeder GK, Liao C, Russ PL, Wolfenden R, Marquez VE. J Org Chem. 2009 Aug 21; 74(16):6212-23.
Three-dimensional structure of the R115E mutant of T4-bacteriophage 2'-deoxycytidylate deaminase. [Biochemistry. 2004]
Three-dimensional structure of the R115E mutant of T4-bacteriophage 2'-deoxycytidylate deaminase.
Almog R, Maley F, Maley GF, Maccoll R, Van Roey P. Biochemistry. 2004 Nov 2; 43(43):13715-23.
The 1.48 A resolution crystal structure of the homotetrameric cytidine deaminase from mouse. [Biochemistry. 2006]
The 1.48 A resolution crystal structure of the homotetrameric cytidine deaminase from mouse.
Teh AH, Kimura M, Yamamoto M, Tanaka N, Yamaguchi I, Kumasaka T. Biochemistry. 2006 Jun 27; 45(25):7825-33.
Review Advances in glycogen phosphorylase inhibitor design. [Curr Opin Investig Drugs. 2008]
Review Advances in glycogen phosphorylase inhibitor design.
Oikonomakos NG, Somsák L. Curr Opin Investig Drugs. 2008 Apr; 9(4):379-95.
Review Cytidine deamination and resistance to retroviral infection: towards a structural understanding of the APOBEC proteins. [Virology. 2005]
Review Cytidine deamination and resistance to retroviral infection: towards a structural understanding of the APOBEC proteins.
Huthoff H, Malim MH. Virology. 2005 Apr 10; 334(2):147-53.

See reviews... See all...

Cited by 9 PubMed Central articles

Delineation of the molecular mechanisms of nucleoside recognition by cytidine deaminase through virtual screening. [ChemMedChem. 2011]
Delineation of the molecular mechanisms of nucleoside recognition by cytidine deaminase through virtual screening.
Costanzi S, Vilar S, Micozzi D, Carpi FM, Ferino G, Vita A, Vincenzetti S. ChemMedChem. 2011 Aug 1; 6(8):1452-8. Epub 2011 May 19.
Role of tyrosine 33 residue for the stabilization of the tetrameric structure of human cytidine deaminase. [Int J Biol Macromol. 2010]
Role of tyrosine 33 residue for the stabilization of the tetrameric structure of human cytidine deaminase.
Micozzi D, Pucciarelli S, Carpi FM, Costanzi S, De Sanctis G, Polzonetti V, Natalini P, Santarelli IF, Vita A, Vincenzetti S. Int J Biol Macromol. 2010 Nov 1; 47(4):471-82. Epub 2010 Jul 14.
Contrasting behavior of conformationally locked carbocyclic nucleosides of adenosine and cytidine as substrates for deaminases. [Nucleosides Nucleotides Nuclei...]
Contrasting behavior of conformationally locked carbocyclic nucleosides of adenosine and cytidine as substrates for deaminases.
Marquez VE, Schroeder GK, Ludek OR, Siddiqui MA, Ezzitouni A, Wolfenden R. Nucleosides Nucleotides Nucleic Acids. 2009 May; 28(5):614-32.

See all...

Structures reported by this article

Crystal Structure Of Human Cytidine Deaminase

PDB: 1MQ0

Source: Homo sapiens

Method: X-Ray Diffraction

Resolution: 2.4 Å

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
BioAssay
PubChem BioAssay experiments on the biological activities of small molecules that cite the current articles. The depositors of BioAssay data provide these references.
BioSystems
Pathways and biological systems (BioSystems) that cite the current articles. Citations are from the BioSystems source databases (KEGG and BioCyc).
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Structure
Three-dimensional structure records in the NCBI Structure database for data reported in the current articles.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Structure of human cytidine deaminase bound to a potent inhibitor.
Structure of human cytidine deaminase bound to a potent inhibitor.
J Med Chem. 2005 Feb 10 ;48(3):658-60.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: