Anal Chem. 2005 Feb 1;77(3):878-85.

LC-DAD-MS/SPE-NMR hyphenation. A tool for the analysis of pharmaceutically used plant extracts: identification of isobaric iridoid glycoside regioisomers from Harpagophytum procumbens.

Seger C, Godejohann M, Tseng LH, Spraul M, Girtler A, Sturm S, Stuppner H.

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Institute of Pharmacy, Department of Pharmacognosy, Leopold Franzens University Innsbruck, Innrain 52, A-6020 Innsbruck, Austria.

Abstract

LC-DAD-MS monitored fractionation of a Harpagophytum procumbens DC. (Pedaliaceae) root extract was combined with a hyphenated LC-DAD-MS/SPE-NMR technique, thus providing the spectral data needed for structure elucidation. This approach allowed the characterization of isobaric iridoid glycoside regioisomers present only as minor constituents. The analytes were identified as the (E/Z) pairs of 6'-O-(p-coumaroyl)harpagide (6'-PCHG) and 8-O-(p-coumaroyl)-harpagide (8-PCHG). The fact that 8-(Z)-PCHG constitutes a new natural product underlines the analytical power of this combined approach. Furthermore, derivatives 6'-(Z)- and 6'-(E)-PCHG are new constituents for H. procumbens.

PMID:: 15679357; [PubMed - indexed for MEDLINE]

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