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J Med Microbiol. 2005 Feb;54(Pt 2):159-62.

Toxigenic status of Clostridium difficile in a large Spanish teaching hospital.

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  • 1Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, C/Doctor Esquerdo, 46, 28007 Madrid, Spain. ralonso.hgugm@salud.madrid.org


The aim of this study was to evaluate the toxigenic status of circulating strains of Clostridium difficile in a large teaching hospital. Overall 220 isolates were studied of which 199 (90.5 %) produced both large clostridial toxins detected by conventional methods. Ten more strains (4.5 %) had toxin A and B genes detectable by PCR. Eleven (5.0 %) variant strains (A- B+) were detected among the isolates studied and 10 strains (4.5 %) had the binary toxin genes (cdtA and cdtB).

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