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    Cloning Stem Cells. 2004;6(3):211-6.

    Highly efficient mRNA-based gene transfer in feeder-free cultured H9 human embryonic stem cells.

    Ponsaerts P, van der Sar S, Van Tendeloo VF, Jorens PG, Berneman ZN, Singh PB.

    Source

    Laboratory of Experimental Hematology, Faculty of Medicine, Antwerp University Hospital, University of Antwerp, Antwerp, Belgium. Peter.Ponsaerts@ua.ac.be

    Abstract

    Several protocols have been described for virus-based gene transfer in human embryonic stem (hES) cells, while efficient non-viral methods are currently non-existing. In this study, we investigated the efficiency of mRNA-based gene transfer in feeder-free cultured H9 hES cells, based on electroporation of in vitro transcribed mRNA encoding the enhanced green fluorescent protein (EGFP). Optimisation of culture and electroporation conditions for feeder-free cultured H9 hES cells resulted a highly pure, transgene-expressing (90% positive cells) H9 hES cell population.

    PMID:
    15671669
    [PubMed - indexed for MEDLINE]

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