The CD200 receptor is a novel and potent regulator of murine and human mast cell function

Holly M Cherwinski; Craig A Murphy; Barbara L Joyce; Mike E Bigler; Yaoli S Song; Sandra M Zurawski; Mehrdad M Moshrefi; Daniel M Gorman; Kathy L Miller; Shuli Zhang; Jonathan D Sedgwick; Joseph H Phillips

doi:10.4049/jimmunol.174.3.1348

The CD200 receptor is a novel and potent regulator of murine and human mast cell function

J Immunol. 2005 Feb 1;174(3):1348-56. doi: 10.4049/jimmunol.174.3.1348.

Authors

Holly M Cherwinski¹, Craig A Murphy, Barbara L Joyce, Mike E Bigler, Yaoli S Song, Sandra M Zurawski, Mehrdad M Moshrefi, Daniel M Gorman, Kathy L Miller, Shuli Zhang, Jonathan D Sedgwick, Joseph H Phillips

Affiliation

¹ DNAX Research Institute, Palo Alto, CA 94304, USA.

PMID: 15661892
DOI: 10.4049/jimmunol.174.3.1348

Abstract

CD200R is a member of the Ig supergene family that is primarily expressed on myeloid cells. Recent in vivo studies have suggested that CD200R is an inhibitory receptor capable of regulating the activation threshold of inflammatory immune responses. Here we provide definitive evidence that CD200R is expressed on mouse and human mast cells and that engagement of CD200R by agonist Abs or ligand results in a potent inhibition of mast cell degranulation and cytokine secretion responses. CD200R-mediated inhibition of FcepsilonRI activation was observed both in vitro and in vivo and did not require the coligation of CD200R to FcepsilonRI. Unlike the majority of myeloid inhibitory receptors, CD200R does not contain a phosphatase recruiting inhibitory motif (ITIM); therefore, we conclude that CD200R represents a novel and potent inhibitory receptor that can be targeted in vivo to regulate mast cell-dependent pathologies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, CD
Antigens, Surface / biosynthesis
Antigens, Surface / immunology
Antigens, Surface / metabolism
Antigens, Surface / physiology*
Bone Marrow Cells / immunology
Bone Marrow Cells / metabolism
Cell Degranulation / immunology
Cells, Cultured
Cytokines / antagonists & inhibitors
Cytokines / metabolism
Down-Regulation / immunology
Fetal Blood / cytology
Fetal Blood / immunology
Fetal Blood / metabolism
Humans
Mast Cells / immunology*
Mast Cells / metabolism*
Membrane Glycoproteins / biosynthesis
Membrane Glycoproteins / immunology
Membrane Glycoproteins / physiology*
Mice
Mice, Inbred C57BL
Orexin Receptors
Receptors, Cell Surface
Receptors, IgE / antagonists & inhibitors
Receptors, IgE / physiology
Skin / cytology
Skin / immunology
Skin / metabolism

Substances

Antigens, CD
Antigens, Surface
CD200 receptor, mouse
CD200R1 protein, human
Cd200r1 protein, mouse
Cytokines
Membrane Glycoproteins
Orexin Receptors
Receptors, Cell Surface
Receptors, IgE
antigens, CD200