lin-17/Frizzled and lin-18 regulate POP-1/TCF-1 localization and cell type specification during C. elegans vulval development

Rashmi Deshpande; Takao Inoue; James R Priess; Russell J Hill

doi:10.1016/j.ydbio.2004.10.020

lin-17/Frizzled and lin-18 regulate POP-1/TCF-1 localization and cell type specification during C. elegans vulval development

Dev Biol. 2005 Feb 1;278(1):118-29. doi: 10.1016/j.ydbio.2004.10.020.

Authors

Rashmi Deshpande¹, Takao Inoue, James R Priess, Russell J Hill

Affiliation

¹ Department of Molecular Genetics, The Ohio State University, 963 Biological Science Building, 484 W. 12th Avenue, Columbus, OH 43210, USA.

PMID: 15649465
DOI: 10.1016/j.ydbio.2004.10.020

Abstract

The Caenorhabditis elegans vulva is comprised of highly similar anterior and posterior halves that are arranged in a mirror symmetric pattern. The cell lineages that form each half of the vulva are identical, except that they occur in opposite orientations with respect to the anterior/posterior axis. We show that most vulval cell divisions produce sister cells that have asymmetric levels of POP-1 and that the asymmetry has opposite orientations in the two halves of the vulva. We demonstrate that lin-17 (Frizzled type Wnt receptor) and lin-18 (Ryk) regulate the pattern of POP-1 localization and cell type specification in the posterior half of the vulva. In the absence of lin-17 and lin-18, posterior lineages are reversed and resemble anterior lineages. These experiments suggest that Wnt signaling pathways reorient cell lineages in the posterior half of the vulva from a default orientation displayed in the anterior half of the vulva.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / growth & development*
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cell Differentiation
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Female
Genes, Helminth
High Mobility Group Proteins / genetics
High Mobility Group Proteins / metabolism*
Immunohistochemistry
Intercellular Signaling Peptides and Proteins / genetics
Intercellular Signaling Peptides and Proteins / metabolism
Mutation
Receptor Protein-Tyrosine Kinases / genetics
Receptor Protein-Tyrosine Kinases / metabolism*
Receptors, G-Protein-Coupled / genetics
Receptors, G-Protein-Coupled / metabolism*
Signal Transduction
Vulva / cytology
Vulva / growth & development
Vulva / metabolism
Wnt Proteins

Substances

Caenorhabditis elegans Proteins
DNA-Binding Proteins
High Mobility Group Proteins
Intercellular Signaling Peptides and Proteins
LIN-17 protein, C elegans
Receptors, G-Protein-Coupled
Wnt Proteins
pop-1 protein, C elegans
LIN-18 protein, C elegans
Receptor Protein-Tyrosine Kinases