Engineering stereocontrol into an aldolase-catalysed reaction

Henry J Lamble; Michael J Danson; David W Hough; Steven D Bull

doi:10.1039/b413255f

Engineering stereocontrol into an aldolase-catalysed reaction

Chem Commun (Camb). 2005 Jan 7:(1):124-6. doi: 10.1039/b413255f. Epub 2004 Nov 23.

Authors

Henry J Lamble¹, Michael J Danson, David W Hough, Steven D Bull

Affiliation

¹ Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, Bath, UK BA2 7AY.

PMID: 15614394
DOI: 10.1039/b413255f

Abstract

A novel thermostable aldolase has been developed for synthetic application, and substrate engineering has been used to induce stereocontrol into aldol reactions of this naturally-promiscuous enzyme.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aldehydes / chemistry
Aldehydes / metabolism*
Biomedical Engineering / methods*
Catalysis
Chromatography, High Pressure Liquid
Enzyme Stability
Fructose-Bisphosphate Aldolase / chemistry
Fructose-Bisphosphate Aldolase / metabolism*
Kinetics
Models, Chemical
Stereoisomerism
Sulfolobus / enzymology
Sulfolobus / genetics

Substances

Aldehydes
3-hydroxybutanal
Fructose-Bisphosphate Aldolase