Warning: The NCBI web site requires JavaScript to function. more...
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.
The tandem affinity purification (TAP) tag technique has been used with success to identify under nondenaturing conditions protein complexes in yeast. The technique can be used in mammalian cells, but we found that the original technique does not yield enough recovery for the identification of proteins when mammalian cells growing in monolayer have to be used. We present here a modified TAP tag technique that allows sufficient recovery of proteins from mouse fibroblasts growing in monolayer cultures. The recovery allows protein identification by mass spectrometry.
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on