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1: J Virol. 2005 Jan;79(1):622-5.Click here to read Click here to read Links

Synchronized infection of cell cultures by magnetically controlled virus.

Haim H, Steiner I, Panet A.

Department of Virology, Hadassah Medical School, The Hebrew University, Jerusalem, Israel. hhaim@md.huji.ac.il

To override the diffusion-limited adsorption step of viral infection, we magnetically synchronized cell attachment. Human immunodeficiency virus type 1-based lentivirus preparations were rendered magnetically reactive by association with magnetite nanoparticles, 50 nm in diameter. Application of a magnetic field resulted in immediate redistribution of the viral inoculum to the cell-associated state and completion of the productive adsorption process within 1 min. Independent of adsorption time, viral concentration, and diffusion rate, infection subsequently progressed by the receptor-mediated entry mechanism. Synchronization of this rate-limiting step of infection may now be applied to analyze isolated events in the viral replication sequence.

PMID: 15596857 [PubMed - indexed for MEDLINE]

PMCID: PMC538731