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Nat Biotechnol. 2005 Jan;23(1):94-101. Epub 2004 Dec 12.

Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.

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  • 1Cell Signaling Technology Inc., 166B Cummings Center, Beverly, Massachusetts 01915, USA.

Abstract

Tyrosine kinases play a prominent role in human cancer, yet the oncogenic signaling pathways driving cell proliferation and survival have been difficult to identify, in part because of the complexity of the pathways and in part because of low cellular levels of tyrosine phosphorylation. In general, global phosphoproteomic approaches reveal small numbers of peptides containing phosphotyrosine. We have developed a strategy that emphasizes the phosphotyrosine component of the phosphoproteome and identifies large numbers of tyrosine phosphorylation sites. Peptides containing phosphotyrosine are isolated directly from protease-digested cellular protein extracts with a phosphotyrosine-specific antibody and are identified by tandem mass spectrometry. Applying this approach to several cell systems, including cancer cell lines, shows it can be used to identify activated protein kinases and their phosphorylated substrates without prior knowledge of the signaling networks that are activated, a first step in profiling normal and oncogenic signaling networks.

PMID:
15592455
[PubMed - indexed for MEDLINE]

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