Zentralbl Bakteriol. 1992 Jan;276(2):196-204.

Core-lipid A on the K40 polysaccharide of Escherichia coli O8:K40:H9, a representative of group I capsular polysaccharides.

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Max-Planck-Institut für Immunbiologie, Freiburg.

Abstract

From the capsular K40 polysaccharide of E. coli O8:K40:H9, a fraction was obtained by gel permeation chromatography which in SDS-PAGE exhibited a ladder-like pattern characteristic of lipopolysaccharides. In Western blots, this fraction reacted with a K40-specific antiserum but not with an O8-specific antiserum. It contained, in addition to the constituents of the K40 polysaccharide (glucuronic acid, glucosamine and serine), glucose, galactose, heptose, and KDO. Mild acid hydrolysis of this fraction liberated a lipid moiety which by chemical analysis was characterized as lipid A. From these results, we conclude that the capsular polysaccharide of E. coli O8:K40:H9 is in part bound to core lipid A. The significance of this finding is discussed.

PMID:: 1559008; [PubMed - indexed for MEDLINE]

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Core-lipid A on the K40 polysaccharide of Escherichia coli O8:K40:H9, a representative of group I capsular polysaccharides.

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Cited by 5 PubMed Central articles

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