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PLoS Biol. 2004 Dec;2(12):e432. Epub 2004 Nov 30.

Replication of Norovirus in cell culture reveals a tropism for dendritic cells and macrophages.

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  • 1Department of Pathology and Immunology, Washington University School of Medicine St. Louis, Missouri, USA.

Abstract

Noroviruses are understudied because these important enteric pathogens have not been cultured to date. We found that the norovirus murine norovirus 1 (MNV-1) infects macrophage-like cells in vivo and replicates in cultured primary dendritic cells and macrophages. MNV-1 growth was inhibited by the interferon-alphabeta receptor and STAT-1, and was associated with extensive rearrangements of intracellular membranes. An amino acid substitution in the capsid protein of serially passaged MNV-1 was associated with virulence attenuation in vivo. This is the first report of replication of a norovirus in cell culture. The capacity of MNV-1 to replicate in a STAT-1-regulated fashion and the unexpected tropism of a norovirus for cells of the hematopoietic lineage provide important insights into norovirus biology.

PMID:
15562321
[PubMed - indexed for MEDLINE]
PMCID:
PMC532393
Free PMC Article

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