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Nat Immunol. 2004 Dec;5(12):1243-50. Epub 2004 Nov 14.

Visualizing dendritic cell networks in vivo.

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  • 1Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA.

Abstract

In the steady state, dendritic cells (DCs) in the lymph node induce T cell tolerance to self antigens. Innate signals trigger the maturation of tissue DCs, which migrate into lymph nodes and activate T cells. To examine DCs in vivo, we produced transgenic mice whose DCs expressed enhanced yellow fluorescent protein. Two-photon microscopy of lymph nodes in live mice showed that most of the steady-state DCs were enmeshed in an extensive network and remained in place while actively probing adjacent T cells with their processes. Mature DCs were more motile than steady-state DCs and were rapidly dispersed and integrated into the sessile network, facilitating their interaction with migrating T cells.

PMID:
15543150
[PubMed - indexed for MEDLINE]
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