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Appl Opt. 2004 Nov 1;43(31):5772-7.

Hyperosmotic chemical agent's effect on in vivo cerebral blood flow revealed by laser speckle.

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  • 1The Key Laboratory of Biomedical Photonics of the Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.


We investigated the influence of a hyperosmotic agent (glycerol) on the normal physiological function of tissue by applying the glycerol in vitro and in vivo to rabbit dura mater to assess the changes in the tissue's optical properties. We used a laser speckle imaging technique to study the effect of epidurally applied glycerol on resting cerebral blood flow (CBF). Our results showed that resting CBF decreased as the transparency of the dura mater increased. The challenges for the design of an optical clearing technique were not only the clearing effects and the duration of the action of the chemical agents but also the influence of the glycerol on the tissue's normal physiological function.

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