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Opt Lett. 2004 Oct 1;29(19):2249-51.

High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging.

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  • 1Department of Physics, Division of Investigative Sciences and Department of Bioengineering, Imperial College London, London, UK. j.requejo@imperial.ac.uk


We report the development of a high-speed wide-field fluorescence-lifetime imaging (FLIM) system that provides fluorescence-lifetime images at rates of as many as 29 frames/s. A FLIM multiwell plate reader and a potentially portable FLIM endoscopic system operating at 355-nm excitation have been demonstrated.

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