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Bioinformatics. 2005 Apr 1;21(7):853-9. Epub 2004 Oct 28.

BACCardI--a tool for the validation of genomic assemblies, assisting genome finishing and intergenome comparison.

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  • 1Universität Bielefeld, Center for Biotechnology (CeBiTec) D-33594 Bielefeld, Germany.



We provide the graphical tool BACCardI for the construction of virtual clone maps from standard assembler output files or BLAST based sequence comparisons. This new tool has been applied to numerous genome projects to solve various problems including (a) validation of whole genome shotgun assemblies, (b) support for contig ordering in the finishing phase of a genome project, and (c) intergenome comparison between related strains when only one of the strains has been sequenced and a large insert library is available for the other. The BACCardI software can seamlessly interact with various sequence assembly packages.


Genomic assemblies generated from sequence information need to be validated by independent methods such as physical maps. The time-consuming task of building physical maps can be circumvented by virtual clone maps derived from read pair information of large insert libraries.

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