Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nat Genet. 2004 Nov;36(11):1231-7. Epub 2004 Oct 24.

Targeted gene alteration in Caenorhabditis elegans by gene conversion.

Author information

  • 1Laboratory of Developmental Immunology, Massachusetts General Hospital, and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02114, USA.

Abstract

Now that some genomes have been completely sequenced, the ability to direct specific mutations into genomes is particularly desirable. Here we present a method to create mutations in the Caenorhabditis elegans genome efficiently through transgene-directed, transposon-mediated gene conversion. Engineered deletions targeted into two genes show that the frequency of obtaining the desired mutation was higher using this approach than using standard transposon insertion-deletion approaches. We also targeted an engineered green fluorescent protein insertion-replacement cassette to one of these genes, thereby confirming that custom alleles of different types can be created in vitro to make the corresponding mutations in vivo. This approach should also be applicable to heterologous transposons in C. elegans and other organisms, including vertebrates.

PMID:
15502826
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Write to the Help Desk