Monolayer-controlled substrate selectivity using noncovalent enzyme-nanoparticle conjugates

Rui Hong; Todd Emrick; Vincent M Rotello

doi:10.1021/ja0461163

Monolayer-controlled substrate selectivity using noncovalent enzyme-nanoparticle conjugates

J Am Chem Soc. 2004 Oct 27;126(42):13572-3. doi: 10.1021/ja0461163.

Authors

Rui Hong¹, Todd Emrick, Vincent M Rotello

Affiliation

¹ Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 01003, USA.

PMID: 15493887
DOI: 10.1021/ja0461163

Abstract

Electrostatic interactions were used to noncovalently conjugate chymotrypsin to gold nanoparticles featuring hybrid tetraethylene(glycol)alkanethiol monolayers terminated with carboxylate groups. This conjugation process greatly alters the substrate selectivity of the adsorbed chymotrypsin, inhibiting the hydrolysis of anionic subtrates without affecting the hydrolysis rate of cationic analogues.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Acetates / chemistry*
Catalysis
Chymotrypsin / chemistry*
Ethylene Glycols / chemistry
Gold / chemistry*
Nanostructures / chemistry*
Oligopeptides / chemistry*
Substrate Specificity
Sulfhydryl Compounds / chemistry

Substances

Acetates
Ethylene Glycols
Oligopeptides
Sulfhydryl Compounds
Gold
Chymotrypsin

Grants and funding

GM 62998/GM/NIGMS NIH HHS/United States