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    Yi Chuan Xue Bao. 2004 Jul;31(7):661-7.

    CDNA cloning of a short isoform of human liver NADP (H) -dependent retinol dehydrogenase/reductase and analysis of its characteristics.

    Du J, Huang DY, Liu GF, Wang GL, Xu XL, Wang B, Zhu L.

    Source

    Department of Cell Biology, China Medical University, Shenyang 110001, China. dujing1106@sohu.com

    Abstract

    In this report we found a new short PCR product when we amplified a 635 bp of NRDR fragment by RT-PCR. With 3'-Race and 5'-Race,we obtained two full-length cDNA sequences from human liver tissue,one 1 261 bp NRDR cDNA,another 1 003 bp NRDR isoform (NRDRiso,GenBank accession number:AY071856). The NRDR gene comprises eight exons and seven introns. The NRDRiso cDNA is produced by alternative splicing of NRDR cDNA, with the removal of 4, 5, 6 exons composed of consecutive 258 bp. The open reading frames of the NRDRiso cDNA predict a single polypeptide of 174 amino acids with the calculated minimum molecular mass of 18.6 kDa.

    PMID:
    15473316
    [PubMed - indexed for MEDLINE]

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