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Genome Biol. 2004;5(10):R84. Epub 2004 Sep 30.

A genome annotation-driven approach to cloning the human ORFeome.

Author information

  • 1The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.

Abstract

We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.

PMID:
15461802
[PubMed - indexed for MEDLINE]
PMCID:
PMC545604
Free PMC Article

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