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1: Genome Biol. 2004;5(10):R83. Epub 2004 Sep 28.Click here to read Click here to read Links

A reverse genetic screen in Drosophila using a deletion-inducing mutagen.

Nairz K, Zipperlen P, Dearolf C, Basler K, Hafen E.

Zoologisches Institut, Universität Zürich, Winterthurerstrasse 190, Zurich CH-8057, Switzerland. nairz@zool.unizh.ch

We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible mutation-detection method based on resolution of PCR fragment-length polymorphisms on standard DNA sequencers is implemented. As the spectrum of HMPA-induced mutations is similar in a variety of organisms, it should be possible to transfer this mutagenesis and detection procedure to other model systems.

PMID: 15461801 [PubMed - indexed for MEDLINE]

PMCID: PMC545603