Analysis of the germination of spores of Bacillus subtilis with temperature sensitive spo mutations in the spoVA operon

FEMS Microbiol Lett. 2004 Oct 1;239(1):71-7. doi: 10.1016/j.femsle.2004.08.022.

Abstract

A Bacillus subtilis strain with a base substitution in the ribosome-binding site of spoVAC was temperature sensitive (ts) in sporulation and spores prepared at the permissive temperature were ts in L-alanine-triggered germination, but not in germination with Ca2+-dipicolinic acid (DPA) or dodecylamine. Spores of a ts spo mutant with a missense mutation in the spoVAC coding region were not ts for germination with l-alanine, dodecylamine or Ca2+-DPA. These findings are discussed in light of the proposal that SpoVA proteins are involved not only in DPA uptake during sporulation, but also in DPA release during nutrient-mediated spore germination.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amines
  • Bacillus subtilis / genetics
  • Bacillus subtilis / physiology*
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Molecular Sequence Data
  • Mutation*
  • Operon*
  • Picolinic Acids
  • Spores, Bacterial / physiology
  • Temperature

Substances

  • Amines
  • Bacterial Proteins
  • Picolinic Acids
  • dipicolinic acid
  • dodecylamine