Biosci Biotechnol Biochem. 2004 Sep;68(9):1849-57.

Isolation of a novel promoter for efficient protein production in Aspergillus oryzae.

Ishida H, Hata Y, Kawato A, Abe Y, Kashiwagi Y.

Source

Research Institute, Gekkeikan Sake Co., Ltd, Fushimi-ku, Kyoto, Japan. h-ishida@gekkeikan.co.jp

Abstract

A novel protein overexpression system of Aspergillus oryzae was constructed. Five promoters which originate from A. oryzae expressed sequence tag (EST) clones in submerged culture were obtained by genome walking. These were subjected to beta-glucuronidase (GUS) reporter assays. The promoter of manganese superoxide dismutase-encoding gene (sodM) showed the most GUS production. The sodM gene was abundantly expressed in submerged culture but little expressed in solid-state culture. The sodM promoter was approximately 3-fold induced by the addition of 0.01% H2O2. Glucoamylase production in A. oryzae using the sodM promoter led to secretion of approximately 1 g/l-broth in Czapek-Dox medium for 3 d. Fucose lectin production in A. oryzae using the sodM promoter led to overexpression as a specific and abundant intracellular protein.

PMID:: 15388959; [PubMed - indexed for MEDLINE]

Free full text

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

J-STAGE, Japan Science and Technology Information Aggregator, Electronic

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Supplemental Content

Save items

Related citations in PubMed

Deletion analysis of the superoxide dismutase (sodM) promoter from Aspergillus oryzae. [Appl Microbiol Biotechnol. 2006]
Deletion analysis of the superoxide dismutase (sodM) promoter from Aspergillus oryzae.
Hisada H, Sano M, Ishida H, Hata Y, Abe Y, Machida M. Appl Microbiol Biotechnol. 2006 Oct; 72(5):1048-53. Epub 2006 Mar 18.
Establishment of a hyper-protein production system in submerged Aspergillus oryzae culture under tyrosinase-encoding gene (melO) promoter control. [Appl Microbiol Biotechnol. 2001]
Establishment of a hyper-protein production system in submerged Aspergillus oryzae culture under tyrosinase-encoding gene (melO) promoter control.
Ishida H, Matsumura K, Hata Y, Kawato A, Suginami K, Abe Y, Imayasu S, Ichishima E. Appl Microbiol Biotechnol. 2001 Oct; 57(1-2):131-7.
Improvement of the glaB promoter expressed in solid-state fermentation (SSF) of Aspergillus oryzae. [Biosci Biotechnol Biochem. 2006]
Improvement of the glaB promoter expressed in solid-state fermentation (SSF) of Aspergillus oryzae.
Ishida H, Hata Y, Kawato A, Abe Y. Biosci Biotechnol Biochem. 2006 May; 70(5):1181-7.
Review Impact of Aspergillus oryzae genomics on industrial production of metabolites. [Mycopathologia. 2006]
Review Impact of Aspergillus oryzae genomics on industrial production of metabolites.
Abe K, Gomi K, Hasegawa F, Machida M. Mycopathologia. 2006 Sep; 162(3):143-53.
Review Genomics of Aspergillus oryzae. [Biosci Biotechnol Biochem. 2007]
Review Genomics of Aspergillus oryzae.
Kobayashi T, Abe K, Asai K, Gomi K, Juvvadi PR, Kato M, Kitamoto K, Takeuchi M, Machida M. Biosci Biotechnol Biochem. 2007 Mar; 71(3):646-70. Epub 2007 Mar 7.

See reviews... See all...

Cited by 3 PubMed Central articles

Display of both N- and C-terminal target fusion proteins on the Aspergillus oryzae cell surface using a chitin-binding module. [Appl Microbiol Biotechnol. 2010]
Display of both N- and C-terminal target fusion proteins on the Aspergillus oryzae cell surface using a chitin-binding module.
Tabuchi S, Ito J, Adachi T, Ishida H, Hata Y, Okazaki F, Tanaka T, Ogino C, Kondo A. Appl Microbiol Biotechnol. 2010 Aug; 87(5):1783-9. Epub 2010 May 25.
Improved protocols for functional analysis in the pathogenic fungus Aspergillus flavus. [BMC Microbiol. 2007]
Improved protocols for functional analysis in the pathogenic fungus Aspergillus flavus.
He ZM, Price MS, Obrian GR, Georgianna DR, Payne GA. BMC Microbiol. 2007 Nov 26; 7:104. Epub 2007 Nov 26.
Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditions. [DNA Res. 2007]
Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditions.
Akao T, Sano M, Yamada O, Akeno T, Fujii K, Goto K, Ohashi-Kunihiro S, Takase K, Yasukawa-Watanabe M, Yamaguchi K, et al. DNA Res. 2007 Apr; 14(2):47-57. Epub 2007 May 31.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Isolation of a novel promoter for efficient protein production in Aspergillus or...
Isolation of a novel promoter for efficient protein production in Aspergillus oryzae.
Biosci Biotechnol Biochem. 2004 Sep ;68(9):1849-57.

PubMed
Structure of the CoA transferase from pig heart to 1.7 A resolution.
Structure of the CoA transferase from pig heart to 1.7 A resolution.
Acta Crystallogr D Biol Crystallogr. 2004 Oct ;60(Pt 10):1717-25. Epub 2004 Sep 23 .

PubMed
Comparison of TFII-I gene family members deleted in Williams-Beuren syndrome.
Comparison of TFII-I gene family members deleted in Williams-Beuren syndrome.
Protein Sci. 2004 Oct ;13(10):2588-99.

PubMed
Selective inhibition of inflammatory gene expression in activated T lymphocytes:...
Selective inhibition of inflammatory gene expression in activated T lymphocytes: a mechanism of immune suppression by thiopurines.
J Pharmacol Exp Ther. 2005 Feb ;312(2):537-45. Epub 2004 Sep 23 .

PubMed
Arteriolar network architecture and vasomotor function with ageing in mouse glut...
Arteriolar network architecture and vasomotor function with ageing in mouse gluteus maximus muscle.
J Physiol. 2004 Dec 1 ;561(Pt 2):535-45. Epub 2004 Sep 23 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: