J Surg Res. 1992 Mar;52(3):276-85.

Differential inhibition of human basal keratinocyte growth to silver sulfadiazine and mafenide acetate.

McCauley RL, Li YY, Poole B, Evans MJ, Robson MC, Heggers JP, Herndon DN.

Source

Skin Tissue Culture Laboratory, Shriners Burns Institute, Galveston, Texas 77550.

Abstract

The impact of topical antimicrobial agents on improving the survival of patients with major thermal injuries is significant. However, the effects of these agents on cells responsible for wound healing has only recently received attention. Fresh human basal keratinocytes were grown in serum-free modified MCDB 153 medium under standard tissue culture conditions. Cells were subsequently exposed to concentrations of silver sulfadiazine and mafenide acetate as low as 1/100 of that used clinically over a period of 5-7 days. Cellular responses documented with hemocytometer cells counts, cellular protein assays, phase-contrast microscopy, and transmission electron microscopy show only severe toxicity to mafenide acetate. Such data imply that inhibition of wound epithelialization is greater with the use of mafenide acetate than with the use of silver sulfadiazine.

PMID:: 1538606; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Elsevier Science

Supplemental Content

Save items

Related citations in PubMed

In vitro toxicity of topical antimicrobial agents to human fibroblasts. [J Surg Res. 1989]
In vitro toxicity of topical antimicrobial agents to human fibroblasts.
McCauley RL, Linares HA, Pelligrini V, Herndon DN, Robson MC, Heggers JP. J Surg Res. 1989 Mar; 46(3):267-74.
Comparison of the effects of commonly used wound agents on epithelialization and neovascularization. [J Am Coll Surg. 1994]
Comparison of the effects of commonly used wound agents on epithelialization and neovascularization.
Kjolseth D, Frank JM, Barker JH, Anderson GL, Rosenthal AI, Acland RD, Schuschke D, Campbell FR, Tobin GR, Weiner LJ. J Am Coll Surg. 1994 Sep; 179(3):305-12.
Cytoprotection of human dermal fibroblasts against silver sulfadiazine using recombinant growth factors. [J Surg Res. 1994]
Cytoprotection of human dermal fibroblasts against silver sulfadiazine using recombinant growth factors.
McCauley RL, Li YY, Chopra V, Herndon DN, Robson MC. J Surg Res. 1994 Apr; 56(4):378-84.
Review Serious silver sulphadiazine and mafenide acetate dermatitis. [Burns. 1995]
Review Serious silver sulphadiazine and mafenide acetate dermatitis.
McKenna SR, Latenser BA, Jones LM, Barrette RR, Sherman HF, Varcelotti JR. Burns. 1995 Jun; 21(4):310-2.
Review Allergic contact dermatitis to mafenide acetate: a case series and review of the literature. [J Drugs Dermatol. 2007]
Review Allergic contact dermatitis to mafenide acetate: a case series and review of the literature.
Firoz EF, Firoz BF, Williams JF, Henning JS. J Drugs Dermatol. 2007 Aug; 6(8):825-8.

See reviews... See all...

Cited by 3 PubMed Central articles

A comparative study of the effects of topical application of Aloe vera, thyroid hormone and silver sulfadiazine on skin wounds in Wistar rats. [Lab Anim Res. 2012]
A comparative study of the effects of topical application of Aloe vera, thyroid hormone and silver sulfadiazine on skin wounds in Wistar rats.
Tarameshloo M, Norouzian M, Zarein-Dolab S, Dadpay M, Gazor R. Lab Anim Res. 2012 Mar; 28(1):17-21. Epub 2012 Mar 21.
Hypochlorous Acid as a Potential Wound Care Agent: Part II. Stabilized Hypochlorous Acid: Its Role in Decreasing Tissue Bacterial Bioburden and Overcoming the Inhibition of Infection on Wound Healing. [J Burns Wounds. 2007]
Hypochlorous Acid as a Potential Wound Care Agent: Part II. Stabilized Hypochlorous Acid: Its Role in Decreasing Tissue Bacterial Bioburden and Overcoming the Inhibition of Infection on Wound Healing.
Robson MC, Payne WG, Ko F, Mentis M, Donati G, Shafii SM, Culverhouse S, Wang L, Khosrovi B, Najafi R, et al. J Burns Wounds. 2007 Apr 11; 6:e6. Epub 2007 Apr 11.
Direct evidence for uptake of intact liposomes encapsulating silver sulfadiazine by cultured human keratinocytes based on combined transmission electron microscopy and X-ray microanalysis. [Antimicrob Agents Chemother. 1...]
Direct evidence for uptake of intact liposomes encapsulating silver sulfadiazine by cultured human keratinocytes based on combined transmission electron microscopy and X-ray microanalysis.
Schaller M, Wurm R, Korting HC. Antimicrob Agents Chemother. 1997 Mar; 41(3):717-9.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Differential inhibition of human basal keratinocyte growth to silver sulfadiazin...
Differential inhibition of human basal keratinocyte growth to silver sulfadiazine and mafenide acetate.
J Surg Res. 1992 Mar ;52(3):276-85.

PubMed
Determination of maternal body composition in pregnancy and its relevance to per...
Determination of maternal body composition in pregnancy and its relevance to perinatal outcomes.
Obstet Gynecol Surv. 2004 Oct ;59(10):731-42; quiz 745-6.

PubMed
Cortical dendritic pathology in human immunodeficiency virus encephalitis.
Cortical dendritic pathology in human immunodeficiency virus encephalitis.
Lab Invest. 1992 Mar ;66(3):285-91.

PubMed
New insights into drug absorption: studies with sirolimus.
New insights into drug absorption: studies with sirolimus.
Ther Drug Monit. 2004 Oct ;26(5):463-7.

PubMed
C-reactive protein is associated with psychological risk factors of cardiovascul...
C-reactive protein is associated with psychological risk factors of cardiovascular disease in apparently healthy adults.
Psychosom Med. 2004 Sep-Oct ;66(5):684-91.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: