J Immunol. 1992 Mar 1;148(5):1451-7.

Chemical cross-linking of class I molecules on cells creates receptive peptide binding sites.

Rock KL, Rothstein L, Gamble S, Gramm C, Benacerraf B.

Source

Division of Lymphocyte Biology, Dana Farber Cancer Institute, Boston, MA 02115.

Abstract

Class I heterodimers on the surface of cells are generally unreceptive to binding peptides in the absence of exogenous beta 2-microglobulin. Paraformaldehyde covalently cross-links beta 2-microglobulin to class I heavy chains in situ and stabilizes empty class I heterodimers. Functionally, this cross-linking creates receptive class I peptide binding sites by acting on beta 2-microglobulin-associated molecules. The presentation of preexisting peptide-class I complexes is also enhanced. These findings support a model whereby a structural alteration, the dissociation of beta 2-microglobulin, limits the existence of receptive class I molecules on normal cells and may control the half-life of active class I molecules.

PMID:: 1538130; [PubMed - indexed for MEDLINE]

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Research Support, U.S. Gov't, P.H.S.

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R01 AI20248/AI/NIAID NIH HHS/United States

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Analysis of the association of peptides of optimal length to class I molecules on the surface of cells. [Proc Natl Acad Sci U S A. 1992]
Analysis of the association of peptides of optimal length to class I molecules on the surface of cells.
Rock KL, Rothstein L, Benacerraf B. Proc Natl Acad Sci U S A. 1992 Oct 1; 89(19):8918-22.
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Cited by 3 PubMed Central articles

Major histocompatibility complex class I presentation of peptides derived from soluble exogenous antigen by a subset of cells engaged in phagocytosis. [J Exp Med. 1995]
Major histocompatibility complex class I presentation of peptides derived from soluble exogenous antigen by a subset of cells engaged in phagocytosis.
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Determinant selection of major histocompatibility complex class I-restricted antigenic peptides is explained by class I-peptide affinity and is strongly influenced by nondominant anchor residues. [J Exp Med. 1994]
Determinant selection of major histocompatibility complex class I-restricted antigenic peptides is explained by class I-peptide affinity and is strongly influenced by nondominant anchor residues.
Chen W, Khilko S, Fecondo J, Margulies DH, McCluskey J. J Exp Med. 1994 Oct 1; 180(4):1471-83.
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