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FEMS Microbiol Lett. 1992 Jan 1;69(2):191-5.

Intraspecific hybridisation of Trichoderma pseudokoningii by anastomosis and by protoplast fusion.

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  • 1Department of Genetics, University of Sao Paulo, Brazil.

Abstract

Double auxotrophic and morphological mutants of Trichoderma pseudokoningii Rifai were fused by anastomosis and by protoplast fusion. The recovery of recombinants from heterokaryons on different selective media and from heterokaryotic colonies indicated the occurrence of parasexual events. Prototrophic colonies growing on minimal medium produced binucleate spores, green in colour, revealing a non-autonomous system for conidial pigmentation. Recombinants were obtained from these dikaryotic colonies suggesting the occurrence of a highly unstable diploid phase.

PMID:
1537549
[PubMed - indexed for MEDLINE]
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