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1: Neuroreport. 2004 Oct 5;15(14):2181-4. Links

Minocycline protects PC12 cells from ischemic-like injury and inhibits 5-lipoxygenase activation.

Song Y, Wei EQ, Zhang WP, Zhang L, Liu JR, Chen Z.

Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310031, China.

Minocycline protects animals against cerebral ischemia by inhibiting inflammation. To determine whether minocycline protects PC12 cells from in vitro ischemic-like injury and affects pro-inflammatory 5-lipoxygenase activation, the cell viability and 5-lipoxygenase translocation to nuclear membrane were observed after oxygen-glucose deprivation (OGD). We found that OGD reduced cell viability, which was attenuated by minocycline and 5-lipoxygenase inhibitor caffeic acid. 5-Lipoxygenase protein was detected in PC12 cells by immunohistochemical and Western blot analyses. OGD induced 5-lipoxygenase translocation to nuclear membranes, which was abolished by minocycline and caffeic acid. Thus, minocycline can protect PC12 cells from in vitro ischemic-like injury, and this effect may be partly related to the inhibition of 5-lipoxygenase activation.

PMID: 15371729 [PubMed - indexed for MEDLINE]