FEBS Lett. 2004 Sep 10;574(1-3):31-6.

Aquaporin homologues in plants and mammals transport ammonia.

Jahn TP, Møller AL, Zeuthen T, Holm LM, Klaerke DA, Mohsin B, Kühlbrandt W, Schjoerring JK.

Source

Plant Nutrition Laboratory, Department of Agricultural Sciences, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. tpj@kvl.dk

Abstract

Using functional complementation and a yeast mutant deficient in ammonium (NH4+) transport (Deltamep1-3), three wheat (Triticum aestivum) TIP2 aquaporin homologues were isolated that restored the ability of the mutant to grow when 2 mM NH4+ was supplied as the sole nitrogen source. When expressed in Xenopus oocytes, TaTIP2;1 increased the uptake of NH4+ analogues methylammonium and formamide. Furthermore, expression of TaTIP2;1 increased acidification of the oocyte-bathing medium containing NH4+ in accordance with NH3 diffusion through the aquaporin. Homology modeling of TaTIP2;1 in combination with site directed mutagenesis suggested a new subgroup of NH3-transporting aquaporins here called aquaammoniaporins. Mammalian AQP8 sharing the aquaammoniaporin signature also complemented NH4+ transport deficiency in yeast.

PMID:: 15358535; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

AMMONIA - HSDB

Supplemental Content

Save items

Related citations in PubMed

NH3 and NH4+ permeability in aquaporin-expressing Xenopus oocytes. [Pflugers Arch. 2005]
NH3 and NH4+ permeability in aquaporin-expressing Xenopus oocytes.
Holm LM, Jahn TP, Møller AL, Schjoerring JK, Ferri D, Klaerke DA, Zeuthen T. Pflugers Arch. 2005 Sep; 450(6):415-28. Epub 2005 Jun 30.
Aquaporin-8-facilitated mitochondrial ammonia transport. [Biochem Biophys Res Commun. 2010]
Aquaporin-8-facilitated mitochondrial ammonia transport.
Soria LR, Fanelli E, Altamura N, Svelto M, Marinelli RA, Calamita G. Biochem Biophys Res Commun. 2010 Mar 5; 393(2):217-21. Epub 2010 Feb 2.
Function of a separate NH3-pore in Aquaporin TIP2;2 from wheat. [FEBS Lett. 2007]
Function of a separate NH3-pore in Aquaporin TIP2;2 from wheat.
Bertl A, Kaldenhoff R. FEBS Lett. 2007 Nov 27; 581(28):5413-7. Epub 2007 Oct 29.
Review Ammonia and urea permeability of mammalian aquaporins. [Handb Exp Pharmacol. 2009]
Review Ammonia and urea permeability of mammalian aquaporins.
Litman T, Søgaard R, Zeuthen T. Handb Exp Pharmacol. 2009; (190):327-58.
Review A look inside: localization patterns and functions of intracellular plant aquaporins. [New Phytol. 2009]
Review A look inside: localization patterns and functions of intracellular plant aquaporins.
Wudick MM, Luu DT, Maurel C. New Phytol. 2009 Oct; 184(2):289-302. Epub 2009 Aug 11.

See reviews... See all...

Cited by 13 PubMed Central articles

Silver ions disrupt K⁺ homeostasis and cellular integrity in intact barley (Hordeum vulgare L.) roots. [J Exp Bot. 2012]
Silver ions disrupt K⁺ homeostasis and cellular integrity in intact barley (Hordeum vulgare L.) roots.
Coskun D, Britto DT, Jean YK, Schulze LM, Becker A, Kronzucker HJ. J Exp Bot. 2012 Jan; 63(1):151-62. Epub 2011 Sep 23.
Depletion of the heaviest stable N isotope is associated with NH4+/NH3 toxicity in NH4+-fed plants. [BMC Plant Biol. 2011]
Depletion of the heaviest stable N isotope is associated with NH4+/NH3 toxicity in NH4+-fed plants.
Ariz I, Cruz C, Moran JF, González-Moro MB, García-Olaverri C, González-Murua C, Martins-Loução MA, Aparicio-Tejo PM. BMC Plant Biol. 2011 May 16; 11:83. Epub 2011 May 16.
Aquaporin biology and nervous system. [Curr Neuropharmacol. 2010]
Aquaporin biology and nervous system.
Barbara B. Curr Neuropharmacol. 2010 Jun; 8(2):97-104.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Aquaporin homologues in plants and mammals transport ammonia.
Aquaporin homologues in plants and mammals transport ammonia.
FEBS Lett. 2004 Sep 10 ;574(1-3):31-6.

PubMed
Role of stress in the pathogenesis of the metabolic syndrome.
Role of stress in the pathogenesis of the metabolic syndrome.
Psychoneuroendocrinology. 2005 Jan ;30(1):1-10.

PubMed
Accumulation of a tetrahydroisoquinoline in phenylketonuria.
Accumulation of a tetrahydroisoquinoline in phenylketonuria.
Science. 1979 Jan 19 ;203(4377):283-4.

PubMed
Molecular biology of severe acute respiratory syndrome coronavirus.
Molecular biology of severe acute respiratory syndrome coronavirus.
Curr Opin Microbiol. 2004 Aug ;7(4):412-9.

PubMed
ACC/AHA guidelines for the management of patients with ST-elevation myocardial i...
ACC/AHA guidelines for the management of patients with ST-elevation myocardial infarction--executive summary. A report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines (Writing Committee to revise the 1999 guidelines for the management of patients with acute myocardial infarction).
J Am Coll Cardiol. 2004 Aug 4 ;44(3):671-719.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: