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Curr Opin Biotechnol. 2004 Aug;15(4):305-13.

Enantioselective biocatalysis optimized by directed evolution.

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  • 1Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Duesseldorf, Forschungszentrum Juelich, D-52426 Juelich, Germany.


Directed evolution methods are now widely used for the optimization of diverse enzyme properties, which include biotechnologically relevant characteristics like stability, regioselectivity and, in particular, enantioselectivity. In principle, three different approaches are followed to optimize enantioselective reactions: the development of whole-cell biocatalysts through the creation of designer organisms; the optimization of enzymes with existing enantioselectivity for process conditions; and the evolution of novel enantioselective biocatalysts starting from non-selective wild-type enzymes.

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