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Genome Biol. 2004;5(9):R72. Epub 2004 Aug 31.

Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae.

Author information

  • 1Unité de Génétique Moléculaire des Levures, URA2171 CNRS and UFR 927 Université Pierre et Marie Curie, Paris-Cedex 15, France. jboyer@pasteur.fr

Abstract

We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.

PMID:
15345056
[PubMed - indexed for MEDLINE]
PMCID:
PMC522879
Free PMC Article

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