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Genome Biol. 2004;5(9):R71. Epub 2004 Aug 17.

A catalog of human cDNA expression clones and its application to structural genomics.

Author information

  • 1Protein Structure Factory, Heubnerweg 6, 14059 Berlin, Germany. buessow@molgen.mpg.de

Abstract

We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.

PMID:
15345055
[PubMed - indexed for MEDLINE]
PMCID:
PMC522878
Free PMC Article

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