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Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13472-7. Epub 2004 Sep 1.

DNA heterogeneity and phosphorylation unveiled by single-molecule electrophoresis.

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  • 1Department of Molecular and Cellular Biology, Biological Laboratories, 16 Divinity Avenue, Harvard University, Cambridge, MA 02138, USA.


Broad-spectrum analysis of DNA and RNA samples is of increasing importance in the growing field of biotechnology. We show that nanopore measurements may be used to assess the purity, phosphorylation state, and chemical integrity of nucleic acid preparations. In contrast with gel electrophoresis and mass spectrometry, an unprecedented dynamic range of DNA sizes and concentrations can be evaluated in a single data acquisition process that spans minutes. Because the molecule information is quantized and digitally recorded with single-molecule resolution, the sensitivity of the system can be adjusted in real time to detect trace amounts of a particular DNA species.

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