Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Protein Sci. 2004 Oct;13(10):2819-24. Epub 2004 Aug 31.

Signal peptide prediction based on analysis of experimentally verified cleavage sites.

Author information

  • 1Department of Bioinformatics, Genentech, Inc., South San Francisco, CA 94080, USA. zemin@gene.com

Abstract

A number of computational tools are available for detecting signal peptides, but their abilities to locate the signal peptide cleavage sites vary significantly and are often less than satisfactory. We characterized a set of 270 secreted recombinant human proteins by automated Edman analysis and used the verified cleavage sites to evaluate the success rate of a number of computational prediction programs. An examination of the frequency of amino acid in the N-terminal region of the data set showed a preference of proline and glutamine but a bias against tyrosine. The data set was compared to the SWISS-PROT database and revealed a high percentage of discrepancies with cleavage site annotations that were computationally generated. The best program for predicting signal sequences was found to be SignalP 2.0-NN with an accuracy of 78.1% for cleavage site recognition. The new data set can be utilized for refining prediction algorithms, and we have built an improved version of profile hidden Markov model for signal peptides based on the new data.

PMID:
15340161
[PubMed - indexed for MEDLINE]
PMCID:
PMC2286551
Free PMC Article

Images from this publication.See all images (2)Free text

Figure 1.
Figure 2.

LinkOut - more resources

Full Text Sources

Other Literature Sources

Research Materials

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for John Wiley & Sons, Inc. Icon for PubMed Central
    Loading ...
    Write to the Help Desk