Nature. 2004 Sep 16;431(7006):333-5. Epub 2004 Aug 25.

Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.

Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang PT, Green-Church KB, Chan MK, Krzycki JA.

Source

Department of Microbiology, 484 West 12th Avenue, The Ohio State University, Columbus, Ohio 43210, USA.

Abstract

Pyrrolysine is the 22nd amino acid. An unresolved question has been how this atypical genetically encoded residue is inserted into proteins, because all previously described naturally occurring aminoacyl-tRNA synthetases are specific for one of the 20 universally distributed amino acids. Here we establish that synthetic L-pyrrolysine is attached as a free molecule to tRNA(CUA) by PylS, an archaeal class II aminoacyl-tRNA synthetase. PylS activates pyrrolysine with ATP and ligates pyrrolysine to tRNA(CUA) in vitro in reactions specific for pyrrolysine. The addition of pyrrolysine to Escherichia coli cells expressing pylT (encoding tRNA(CUA)) and pylS results in the translation of UAG in vivo as a sense codon. This is the first example from nature of direct aminoacylation of a tRNA with a non-canonical amino acid and shows that the genetic code of E. coli can be expanded to include UAG-directed pyrrolysine incorporation into proteins.

Comment in

Nature. 2004 Sep 16;431(7006):257-8.

PMID:: 15329732; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

L-LYSINE - HSDB

Supplemental Content

Save items

Related citations in PubMed

Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA. [Science. 2002]
Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA.
Srinivasan G, James CM, Krzycki JA. Science. 2002 May 24; 296(5572):1459-62.
Specificity of pyrrolysyl-tRNA synthetase for pyrrolysine and pyrrolysine analogs. [J Mol Biol. 2009]
Specificity of pyrrolysyl-tRNA synthetase for pyrrolysine and pyrrolysine analogs.
Li WT, Mahapatra A, Longstaff DG, Bechtel J, Zhao G, Kang PT, Chan MK, Krzycki JA. J Mol Biol. 2009 Jan 30; 385(4):1156-64. Epub 2008 Nov 25.
Review The direct genetic encoding of pyrrolysine. [Curr Opin Microbiol. 2005]
Review The direct genetic encoding of pyrrolysine.
Krzycki JA. Curr Opin Microbiol. 2005 Dec; 8(6):706-12. Epub 2005 Oct 26.
The complete biosynthesis of the genetically encoded amino acid pyrrolysine from lysine. [Nature. 2011]
The complete biosynthesis of the genetically encoded amino acid pyrrolysine from lysine.
Gaston MA, Zhang L, Green-Church KB, Krzycki JA. Nature. 2011 Mar 31; 471(7340):647-50.
Review Genetic code: introducing pyrrolysine. [Curr Biol. 2002]
Review Genetic code: introducing pyrrolysine.
Ibba M, Söll D. Curr Biol. 2002 Jul 9; 12(13):R464-6.

See reviews... See all...

Cited by 25 PubMed Central articles

Site-specific protein modifications through pyrroline-carboxy-lysine residues. [Proc Natl Acad Sci U S A. 2011]
Site-specific protein modifications through pyrroline-carboxy-lysine residues.
Ou W, Uno T, Chiu HP, Grünewald J, Cellitti SE, Crossgrove T, Hao X, Fan Q, Quinn LL, Patterson P, et al. Proc Natl Acad Sci U S A. 2011 Jun 28; 108(26):10437-42. Epub 2011 Jun 13.
Stereochemical basis for engineered pyrrolysyl-tRNA synthetase and the efficient in vivo incorporation of structurally divergent non-native amino acids. [ACS Chem Biol. 2011]
Stereochemical basis for engineered pyrrolysyl-tRNA synthetase and the efficient in vivo incorporation of structurally divergent non-native amino acids.
Takimoto JK, Dellas N, Noel JP, Wang L. ACS Chem Biol. 2011 Jul 15; 6(7):733-43. Epub 2011 May 5.
The complete biosynthesis of the genetically encoded amino acid pyrrolysine from lysine. [Nature. 2011]
The complete biosynthesis of the genetically encoded amino acid pyrrolysine from lysine.
Gaston MA, Zhang L, Green-Church KB, Krzycki JA. Nature. 2011 Mar 31; 471(7340):647-50.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Protein Clusters
Clusters of related proteins from the Protein Clusters database that cite the current articles. Sources of references in Protein Clusters include the associated Gene and Conserved Domain records as well as NCBI added citations.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Domains
Conserved Domain Database (CDD) records that cite the current articles. Citations are from the CDD source database records (PFAM, SMART).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.
Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.
Nature. 2004 Sep 16 ;431(7006):333-5. Epub 2004 Aug 25 .

PubMed
Community-associated methicillin-resistant Staphylococcus aureus infections in P...
Community-associated methicillin-resistant Staphylococcus aureus infections in Pacific Islanders--Hawaii, 2001-2003.
MMWR Morb Mortal Wkly Rep. 2004 Aug 27 ;53(33):767-70.

PubMed
[Ergonomic aspects of sitting].
[Ergonomic aspects of sitting].
Fortschr Med. 1992 Jan 20 ;110(1-2):29-30, 33.

PubMed
Mosquito larvicidal properties of essential oil of an indigenous plant, Ipomoea ...
Mosquito larvicidal properties of essential oil of an indigenous plant, Ipomoea cairica Linn.
Jpn J Infect Dis. 2004 Aug ;57(4):176-7.

PubMed
Resveratrol-associated renal toxicity.
Resveratrol-associated renal toxicity.
Toxicol Sci. 2004 Dec ;82(2):614-9. Epub 2004 Aug 25 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.

Source

Abstract

Comment in

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Cited by 25 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information