Diseases due to nontuberculous mycobacteria are increasing in frequency, especially in patients with compromised immunity. A number of "new" mycobacterial species have been described in the last decade, largely as the result of the use of new tools to identify previously unrecognized mycobacteria found in the environment and in clinical specimens. This article reviews many of these potentially pathogenic organisms, summarizing what is known regarding their phenotypic and genotypic characterization, antimicrobial susceptibility, and clinical significance.