Chem Biol. 2004 Aug;11(8):1081-91.

Design and validation of a bifunctional ligand display system for receptor targeting.

Chen L, Zurita AJ, Ardelt PU, Giordano RJ, Arap W, Pasqualini R.

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The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA.

Abstract

Here we developed a bacteriophage display particle designed to serve as a bifunctional entity that can target tumors while delivering an agent. We engineered a chimera phage vector containing a pIII-displayed alphav integrins-targeting moiety and a pVIII-displayed streptavidin binding adaptor moiety. By using the chimeric phage particle, targeting of alphav integrins on cells in culture and tumor-related blood vessels was shown through different applications, including luminescent quantum dots localization, surface plasmon resonance-based binding detection, and an in vivo tumor model. The strategy validated here will accelerate the discovery and characterization of receptor-ligand binding events in high throughput, and cell-specific delivery of diagnostics or therapeutics to organs of choice without the need for chemical conjugation.

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Chem Biol. 2004 Aug;11(8):1033-5.

PMID:: 15324809; [PubMed - indexed for MEDLINE]

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