P

_{f} dynamics. A and A′, The time courses of volume during hypotonic shock of 40 mOsm, 100 mOsm, and 200 mOsm (means ±

se, symbols without connecting lines) were fitted with model 5 (for clarity, omitting data fitted with model 6). The fitted record durations varied between 15 s, 32 s, and 45 s (in record duration we refer strictly to the net duration of the post-delay swelling period; the delay was also included in the fit irrespective of its duration). P

_{f} values were plotted (means ±

se, symbols connected by lines) depending on the fitted record duration, as follows: the P

_{f} at the beginning and the end of each delay was plotted at 0

*μ*m s

^{−1} (according to model 5), then the best-fit

of the 15-s record was plotted at the end of the best-fit delay, marking a step increase of P

_{f} (see model 5, Fig. 4A). The final (end-of-the-record) P

_{f} values of all records,

, were calculated using the corresponding best-fit

(Eq. 6, Supplemental Appendix I) and were placed at the time points corresponding to the end of the fitted records (record length of 15 s, 32 s or 45 s + delay; the differences between the horizontal positions of the symbols reflect the different durations of the best-fit delays). The results were grouped, as indicated, into low-

cells and high-

cells, based on their 15-s record

values. B and B′, The corresponding time courses of bath perfusion (Eq. 1b, with the following values: for delC of 200 mOsm: as in Fig. 1A, b; for delC of 100 mOsm: t

_{width} = 2.5 s, t

_{half} = −34.4 s, lag = 1 s,

,

, and

), and for delC of 40 mOsm: t

_{width} = 2.5 s, t

_{half} = −34.4 s, lag = 1 s,

,

, and

). C and C′, The best-fit values of delay extracted from the 15-s-long records (mean ±

se), as listed in Supplemental Table II. D and D′, The best-fit values of

extracted from the 15-s-long records (mean ±

se). #, Significant difference from zero (

*P* < 0.05).

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