Induction of neutralising antibodies restricts the use of human granulocyte/macrophage colony stimulating factor for vaccine studies in rhesus macaques

Vaccine. 2004 Sep 3;22(25-26):3295-302. doi: 10.1016/j.vaccine.2004.03.002.

Abstract

Granulocyte/macrophage-colony stimulating factor (GM-CSF) is a valuable adjuvant to enhance induction of cellular immune responses in rodents. Less information is available regarding its use as an adjuvant in primates or humans. We explored recombinant human GM-CSF for potential vaccine studies in rhesus macaques and focused on its effect on peripheral monocytes as progenitors of dendritic cells and its potential immunogenicity. Application of human GM-CSF to nine animals led to an average 32-fold increase in monocyte numbers. This was not observed upon re-treatment, which coincided with GM-CSF-specific neutralising antibodies. These also neutralised the activity of rhesus macaque GM-CSF. The data underscore the need to use species-specific GM-CSF for immunomodulation in primates.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Blocking / biosynthesis*
  • Antibodies, Blocking / immunology*
  • Blotting, Western
  • Cell Separation
  • Cloning, Molecular
  • Cytokines / pharmacology
  • Female
  • Granulocyte-Macrophage Colony-Stimulating Factor / antagonists & inhibitors*
  • Granulocyte-Macrophage Colony-Stimulating Factor / immunology*
  • Humans
  • Macaca mulatta
  • Male
  • Monocytes / immunology
  • Neutralization Tests
  • Vaccines / immunology*

Substances

  • Antibodies, Blocking
  • Cytokines
  • Vaccines
  • Granulocyte-Macrophage Colony-Stimulating Factor