Format

Send to:

Choose Destination
See comment in PubMed Commons below
Hum Reprod. 2004 Oct;19(10):2319-24. Epub 2004 Aug 6.

Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover.

Author information

  • 1Department of Reproductive Medicine, St Mary's Hospital, Manchester M13 OJH, UK. Daniel.Brison@man.ac.uk

Abstract

BACKGROUND:

IVF is limited by low success rates and an unacceptably high multiple pregnancy rate. These outcomes would be improved significantly if a single embryo of high viability could be replaced in each treatment cycle, but widespread acceptance of such a policy is hindered by the lack of predictive factors for embryo selection. We have conducted a retrospective clinical study of a novel non-invasive method of embryo selection based on the depletion/appearance of amino acids in the culture medium.

METHODS:

Fifty-three cycles of IVF treatment using ICSI were studied. Embryos were cultured for 24 h in 4 microl drops of medium containing a physiological mixture of 18 amino acids. The spent medium was analysed for amino acid content by high performance liquid chromatography.

RESULTS:

The turnover of three amino acids, Asn, Gly and Leu, was significantly correlated with a clinical pregnancy and live birth. These correlations were independent of known predictors, such as female age, basal levels of FSH, embryo cell number and embryo morphological grade.

CONCLUSIONS:

Non-invasive assay of amino acid turnover has the potential to improve significantly the prospective selection of the most viable embryos, or single embryo, for replacement in an IVF cycle.

PMID:
15298971
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Write to the Help Desk