Abstract
Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis alpha-amylase by using the C-terminal-half region of alpha-agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins. In 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5% of theoretical yield from raw corn starch.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Culture Media
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Ethanol / metabolism*
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Fermentation
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Gene Expression Regulation, Fungal
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Genetic Engineering / methods*
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Glucan 1,4-alpha-Glucosidase / genetics
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Glucan 1,4-alpha-Glucosidase / metabolism*
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Industrial Microbiology / methods
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Plasmids
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Recombinant Proteins
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Rhizopus / enzymology
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Rhizopus / genetics
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
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Starch / metabolism*
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Streptococcus bovis / enzymology
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Streptococcus bovis / genetics
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Zea mays / chemistry
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Zea mays / metabolism*
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alpha-Amylases / genetics
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alpha-Amylases / metabolism*
Substances
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Culture Media
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Recombinant Proteins
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Ethanol
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Starch
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alpha-Amylases
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Glucan 1,4-alpha-Glucosidase